ABSTRACT
Objective To explore tumor inhibitory effect of the lentiviral vectors mediated small interfering RNA(siRNA)targe‐ting survivin gene on human lung adenocarcinoma xenografts in nude mice in vivo .Methods The lentiviral vector expressing sur‐vivin‐siRNA was prepared .Then established human lung adenocarcinoma‐bearing nude mice model ,and divided nude mice into three groups ,including the blank control group(PC group) ,blank vector group(NC group) and experiment group(RNAi group) ,treated with physiological saline ,blank viral vector and siRNA respectively .The lentiviral vectors expressing survivin‐siRNA were locally injected in tumor issues of nude mice in the RNAi group .Then observed the curve about changes of tumor volume in different time . The expression levels of protein and mRNA were detected by using Western blot and reverse transcription polymerase chain reac‐tion(RT‐PCR) .The histogram of tumor cell cycle were examined by using flow cytometry .Results The rate of tumor inhibition of lentiviral vectors mediated survivin‐siRNA on lung adenocarcinoma in nude mice was 46 .07% .There were significant differences in tumor weight between siRNA group and NC group ,PC group(P0 .05) .Compared with NC group and PC group ,the expression levels of survivin mRNA and protein were decreased ,and rates of inhibition were 72 .00% and 53 .00% respectively .The percentage of G1 phase cells was in‐creased ,whereas the percentages of S phase cells was decreased .Conclusion The lentiviral vectors mediated siRNA could effective‐ly inhibit survivin gene expression on human lung adenocarcinoma xenografts in nude mice and markedly induce the apoptosis of tumor .
ABSTRACT
<p><b>OBJECTIVE</b>To explore the clinical efficacy of early application of sequential gastrointestinal lavage in patients with acute paraquat poisoning by analyzing the clinical data of 97 patients.</p><p><b>METHODS</b>A total of 97 eligible patients with acute paraquat poisoning were divided into conventional treatment group (n = 48) and sequential treatment group (n = 49). The conventional treatment group received routine gastric lavage with water. Then 30 g of montmorillonite powder, 30 g of activated charcoal, and mannitol were given to remove intestinal toxins once a day for five days. The sequential treatment group received 60 g of montmorillonite powder for oral administration, followed by small-volume low-pressure manual gastric lavage with 2.5%bicarbonate liquid. Then 30 g of activated charcoal, 30 g of montmorillonite powder, and polyethylene glycol electrolyte lavage solution were given one after another for gastrointestinal lavage once a day for five days. Both groups received large doses of corticosteroids, blood perfusion, and anti-oxidation treatment. The levels of serum potassium, serum amylase (AMY) alanine aminotransferase (ALT), total bilirubin (TBIL), blood urea nitrogen (BUN), creatinine (Cr), lactate (Lac), and PaO₂of patients were determined at 1, 3, 5, 7, and 10 days. Laxative time, mortality, and survival time of dead cases were evaluated in the two groups.</p><p><b>RESULTS</b>The incidence rates of hypokalemia (<3.5 mmol/L) and AMY (>110 U/L) were significantly lower in the sequential treatment group than in the conventional treatment group (P < 0.05). There were no significant differences in the incidence of ALT (>80 U/L), TBIL (>34.2 µmol/L), BUN (>7.2 mmol/L), and Cr (>177 µmol/L) between the two groups (P>0.05). However, the highest levels of ALT, TBIL, BUN, Cr, and Lac were significantly lower in the sequential treatment group than in the conventional treatment group (P < 0.05). Moreover, the sequential treatment group had significantly lower incidence of PaO₂(<60 mmHg), shorter average laxative time, lower mortality, and longer survival time of dead cases than the conventional treatment group (P < 0.05).</p><p><b>CONCLUSION</b>The early application of sequential gastrointestinal lavage can shorten laxative time, alleviate organ damage in the liver, kidney, lung, and pancreas, reduce mortality, and prolong the survival time of dead cases in patients with acute paraquat poisoning.</p>